Cats off duty

I think I am just going to be a cat this weekend. Lazy lying around on a comfortable spot and thinking of nothing much. Maybe eating a bit of high-quality meat now and then, who knows.

Cat resting on blue blanket

And I deserve it, that is the least you can say! Not only did I this week finally clear out the story I had been working on for months, but I also managed to make a first break-through for my néxt modelling paper only this friday afternoon!

Cat enjoying the petting

Three summers ago, when I was working on my master thesis in the Norwegian mountains, I noticed peculiar patterns in the distribution of several alpine species along the roads. As it was not my biggest concern at the time, and as I could not find a convenient way to highlight the patterns in my dataset, the observation did not result in any significant conclusions.

Black cat hiding in cooler

But I never forgot about it, and after the submission of my second paper a month ago, I could give this question priority again. And now, this week, finally, the issue got solved! The story is now send out to my supervisors, ready to face some critical reviews, but until these are back, I am just gonna lay down on this soft blue blanket and close my eyes.

Cat sleeping on blue blanket

But only after I yawned away that freshly catched little bird. Enjoy your weekend!

Spanish feral cat

Well-deserved cat-massage

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To the test

Remember my magical measuring devices for logging soil temperature over long time periods? Remember how excited I was about how cheap, practical, strong and easy to use they are?

iButtons, endless rows of iButtons!

iButtons, endless rows of iButtons!

I still am as excited, but I was however pondering about a few little concerns. As these tiny temperature sensors were originally designed to insure the cold chain of cooled food products in the food industry, they are not perfectly adapted for life in the soil. Their main limitation is their seal, which is not a hundred percent waterproof. This means they need some protection to survive the harsh times below the soil surface.

iButtons with different protection methods

I now already have a lot of experience with iButtons within a little rain coat (as displayed in the picture), and one by one they safely delivered their valuable data. Success ratio: 96 %. This thanks to the combined powers of parafilm and a little ziplock plastic bag.

The iButtons have to help me discover the effect of such slopes on mountain plant life.

The iButtons have to help me discover the effect of such slopes on mountain plant life.

Protection works perfectly, that should be clear, but there is one remaining issue: how big is the effect of this packaging on the measured temperature? Layers of packed air and protective foil dampen the temperature measurements, buffering the peaks .

Left - no protection, right - protection Black: no protection, dark orange: parafilm, licht orange: parafilm + ziploc bag.

Left – no protection, right – protection
Black: no protection, dark orange: parafilm, licht orange: parafilm + ziploc bag.

So I performed a tiny test to measure the effect of our protective coat. First, as a control treatment, I put three iButtons in a cooler without any protection, to see if they indeed measure exactly the same temperature and to get an estimation of their inherent lagging time (graph on the left). You can see there is some small variation, especially at the end of the measurement, showing temporal errors in the temperature recordings. But more importantly, you see that iButtons lag behind in measuring abrupt temperature shocks, due to the way in which they are designed. They need almost half an hour to track the changes and refind a stable level. Keep in mind however that such abrupt temperature changes are pretty rare in the soil, making these lags acceptable in hourly measurements.

iButton lighting up

On the right, I repeated the cooling experiment, but now with packed iButtons. The red dots indicate the iButton wrapped in parafilm, the orange ones an iButton with both parafilm and a plastic bag. By adding the protection, the previously noticed lagging increases, but the difference never gets bigger than two or three minutes.

How does this little brave Saxifraga experience its environment? The iButtons will tell!

How does this little brave Saxifraga experience its environment? The iButtons will tell!

The protected iButtons almost manage to catch up as the temperature starts levelling off, although there lowest extreme value is still 0,2 °C lower than in the unprotected iButton. Note as well that the difference between the two protecting methods is negligible, which gives me reason to use the combined packing method for safety reasons.

I can be at ease in the mountains, the iButtons do what they have to do.

I can be at ease in the mountains, the iButtons do what they have to do.

Conclusion: we see how iButtons lag behind in their measurements, an issue that increases even more when put in protective parafilm. However, as we measure only once every hour, and as real life temperature changes are slower, these lags have a negligible effect on the end result in ecological long-term studies.

We can hence have faith in our iButtons!

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Presenting

Presentation

 

I am presenting intermediate results of my PhD-research at several occassions the last few weeks. Exciting stuff!

 

 

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Out you go!

Little brave seedlings, surviving everything they have to go through! At least until now…

Brave dandelion seedling

Remember the seeds we planted earlier? They were growing so successfully and swift, I decided they were ready for action. They even survived transplantation in separate little pots, followed by cold adaptation to the grey outside conditions of early december, all with almost a hundred percent survival rate.

Plant seedlings in plant tray

 So last week was the best moment to start with the real action and plant them in the gaps in our experimental grassland. We had one nice and sunny day this week that served as the perfect opportunity to put them out.

Experimental vegetation gap

Which is where they are now, ready to face the real winter. Unfortunately, we already had some crazy thunderstorms in the second half of the week. I did not have the chance yet to check how our little friends reacted to these massive downpours, but it would be a pity if all of them washed away on the high tides…

Abstract seedlings

Scary wait, it is, at least till next monday!

Experimental vegetation gap

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Stress

No, no PhD-stress here, or not more than just a little. I have been teaching a practical course on the measurement of plant stress to our master students. They are asked to study stress levels in plant leaves with the use of two old but impressive measuring devices that capture leaf fluorescence.

 PAM-2000 fluorescence measurement

Yes, I am really talking about fluorescence in which objects emit light, even when the light source is already gone. No, this does not result in leaves glooming like eyes of a cat in the dark, unfortunately. Leaves send out light at a wavelength invisible for the eye (but not for my old but impressive devices): infrared.

 Dark adaption clip fluorescence

In the practical course, students find out that the fluorescence of a leaf is the result of light being absorbed by the leaf that cannot be used by photosynthesis. It serves hence an indirect way of measuring how much light energy the plants can use, which is then a measure for their health level.

 Plant efficiency analyser fluorescence

With an ingenuous sequence of light pulses to activate and deactivate the photosynthesis apparatus of the leave, we can find out how much a plant is suffering.

 Dark adaption clips and infrared light source

I for my part, learned a lot of this practical course, of the experiment as well as the teaching experience. Let me see next weeks if the same holds true for the students as soon as they start handing in their reports…

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Now we’re talking

On the greyest and coldest day of the winter so far, I put on my warmest ‘Arctic’ clothing to start a new project.

Measuring microclimate in gaps

We went out into the field to start a project I had been thinking about for a long time already: measuring microclimate with a thermal camera in a truly useful way.

DSC_0006

The thermal camera had been great for illustrative purposes, as it gives beautifully coloured images that spark the imagination, like the one of this reindeer in Lapland (from my archives). However, its use in the field was limited by the difficulty to get long-term data from exactly the same spot, in such a way that it would stay comparable over time.

Thermal reindeer

But now, with the help of a tripod to keep the camera (heavy stuff, I tell you) perpendicular to the ground, I can make movies of daily temperature shifts on whichever small scale I want.

Thermal camera in the field

This cold and grey December day in Belgium, with temperatures staying between – 1 and 1 °C, was in any case the most boring day ever to try this new device. The possibilities look highly promising for the future, however, so there are certainly reasons to celebrate!

Thermal mountains

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